Web4 sep. 2024 · A small fragment library for single-end sequencing was constructed based on the IonS5 TM XL sequencing platform, and clean reads with high quality were obtained through the cutting and filtering of the initial reads. Uparse software (version v7.0.1001, Edgar, Tiburon, CA, ... Web25 sep. 2024 · Changes in the composition and abundance of microbiomes in alveolar lavage fluid from eleven 4-week-old Chinese Kele piglets (three clinically healthy and eight diseased) were studied by IonS5 TM XL sequencing of the bacterial 16S rRNA genes. Results: Histopathological sections showed that diseased pigs displayed more lung …
Investigating the microbiota of fermented fish products
Websamples was performed on an IonS5TMXL sequencer by Novogene Co., Beijing, China. The extracted high-quality sequences were analyzed using the Quantitative Insights into Microbial Ecology (QIIME) software (version 1.7.0). The OTU (operational taxonomic unit) clustering of eective sequences was performed by Uparse software Web20 feb. 2024 · The DNA libraries was generated based on an IonS5TMXL (Thermo Fisher Scientific) (NegNE no. 1–5, SsNE no. 1–5, NegST no. 1–5, and SsST no.1-2) and the Illumina Novaseq 6000 platform (Illumina, USA) (SsST no. 3–5), and quantified via Qubit and Q-PCR. Single-end and paired-end raw reads were generated. raw marrow bones shipped
Effects of respiratory disease on Kele piglets lung microbiome ...
Web10 mrt. 2024 · The sequencing library was constructed using the Ion Plus Fragment Library Kit 48 rxns (Thermo Scientific). After the constructed library was qualified by Qubit … WebAfter the Ion S5™ or Ion S5™ XL System (Cat. nos. A27211 or A27213 ) is uncrated, installation and testing of the Ion S5™ Sequencer or Ion S5™ XL Sequencer and Torrent Server (required for use with the Ion S5™ XL Sequencer) takes ~8 hrs. The Ion Chef™ Instrument or Ion OneTouch™ 2 Instrument plus Ion OneTouch™ ES raw marinated shrimp