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WebHarvested cells were lysed and incubated with anti-Flag M2 affinity gel (A2220, Sigma Aldrich, USA) at 4 °C overnight. The immunoprecipitated proteins were resolved by gel electrophoresis, and the gel was silver-stained using a Pierce Silver Stain Kit (Thermo Scientific, USA) according to the manufacturer's instructions. WebProducts search. 0. Products search hightown va weather
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